Books Protein Design: Methods and Applications (Methods in Molecular Biology, 1216) 2nd ed. 2014 Edition by Valentin Köhler (Editor

"Protein Design: Methods and Applications (2nd Edition, 2014)" edited by Valentin Köhler is a comprehensive guide to the principles, techniques, and protocols used in protein engineering and design. The book offers practical guidance for approaching protein design projects, focusing on creating well-folded peptide structures and redesigning existing proteins for applications in medicine, material science, catalysis, synthetic biology, and molecular evolution studies.

This edition expands on the first with new chapters covering metals as structural and functional elements, fluorinated proteins, symmetric protein fold design, protein libraries, and computational approaches. It includes step-by-step protocols, experimental tips, and troubleshooting advice. Specialized topics such as the use of scaffold search programs for design, global sequence optimization algorithms, and computational redesign of metalloenzymes are also discussed.